Concentrating on Chromatography

Connor Johnson, a researcher from the University of Alberta, discusses his award-winning honours project analyzing the volatile organic compounds (VOCs) in two banana species using headspace gas chromatography-mass spectrometry (HS-GC-MS). He completed this specific project as an undergraduate at Thompson Rivers University (TRU).For over 60 years, commercial banana flavoring has remained unchanged—even though the fruit it's supposed to mimic changed in the 1950s. Connor's research reveals why fake banana tastes fake: the commercial banana extract contains only 3 compounds compared to 18+ in real bananas, missing critical compounds that create authentic banana flavor.This episode covers:- The history of ba...

Join us for an in-depth conversation with Holly Lee, Global Technical Marketing Specialist at SCIEX, as we explore the cutting edge of food and environmental safety testing. Holly shares her expertise on analyzing ultra-short chain PFAS like TFA, masked mycotoxins, pesticide residues, and the latest LC-MS/MS workflows transforming laboratory efficiency.In this episode of Concentrating on Chromatography, we discuss:PFAS Analysis & Challenges- Why ultra-short chain PFAS compounds like trifluoroacetic acid (TFA) are among the toughest analytes to detect- Background contamination issues and how labs can minimize them- Column selection strategies and method development for comprehensive PFAS panels- Combining...

In this episode of Concentrating on Chromatography, we sit down with Alex Brody, an organic chemist at Aqua America, to discuss the rigorous analytical methods required for regulated drinking water testing.Alex walks us through the multi-step PFAS detection process using EPA Method 537.1, including extraction, nitrogen blowdown concentration, and LC-MS analysis. He explains why sample preparation and quality control are critical for achieving trace-level detection—and why these methods can't be rushed or simplified, even with new technologies available.We also explore taste and odor investigations, disinfection byproducts, and volatile organic compounds, plus the surprising reality of why regulated la...

In this episode of Concentrating on Chromatography, we sit down with Andreas Metousis, a PhD researcher at the Max Planck Institute of Biochemistry, to explore cutting-edge spatial proteomics and its role in understanding ovarian cancer development.Andreas discusses how Deep Visual Proteomics (DVP)—a method combining artificial intelligence, laser micro-dissection, and advanced mass spectrometry—is revolutionizing cancer research by providing unprecedented insight into the earliest molecular events in disease progression.Key Topics Covered:- How transcriptomics and proteomics differ and why both matter for cancer research- Deep Visual Proteomics (DVP): AI-driven cell identification and high-resolution protein analysis- Why high-throughput auto...

In this episode of Concentrating on Chromatography, David interviews his former high school chemistry teacher, Dr. Kristen Vanderveen from The Bromfield School, about teaching separation science at the high school level.Dr. Vanderveen shares her approach to introducing chromatography, filtration, and gravimetric analysis to both first-year chemistry students and AP Chemistry students. She discusses the hands-on experiments that work best in a high school setting, including paper chromatography with food dyes, TLC separations, and the challenges of balancing lab time with curriculum requirements.Topics discussed:- Separation methods taught in high school chemistry (filtration, paper chromatography, TLC)- Gravimetric...

Join host David Oliva from Organomation for an in-depth conversation with Sean Orlowicz, Principal Marketing Development Manager at Phenomenex, about the analytical challenges and innovative solutions for GLP-1 peptide therapeutics like semaglutide and tirzepatide.In this episode, presented in collaboration with Separation Science (https://www.sepscience.com/)—a premier learning platform for analytical scientists—Sean shares over 22 years of chromatography expertise covering critical topics including:Key Discussion Topics:- Unique analytical challenges in separating GLP-1 analogues and related impurities- Column selectivity and efficiency optimization for complex peptide separations- The role of Aeris Peptide XB-C18 and core-shell particle technology- Method robu...

Join us for an insightful conversation with Dr. Ryland Giebelhaus a chromatographer and metabolomics researcher from the University of Victoria, as he breaks down fecal microbiota transplantation (FMT)—a revolutionary treatment changing lives by restoring healthy gut bacteria. In this episode, you’ll learn:- What FMT is and why it’s a game-changer for treating recurrent Clostridioides difficile infections and other gut-related diseases - How cutting-edge techniques like comprehensive two-dimensional gas chromatography (GC×GC-TOFMS) enable detection of key metabolites such as short-chain fatty acids, linking gut microbiome function to health - Practical challenges of analyzing biological samples, including sample...

Join us in this fascinating episode of Concentrating on Chromatography as Dr. Rachael Guenter, PhD, shares her eight-year journey researching neuroendocrine tumors. Discover how her background in plant pathology and engineering shaped her translational medicine approach, and learn about the crucial role of mass spectrometry in uncovering protein-level insights in these rare cancers. Dr. Guenter also discusses the challenges of translating lab discoveries into clinical trials, the promise of spatial mass spectrometry for tumor heterogeneity, and the importance of collaboration between scientists, clinicians, and patients. Whether you're interested in cancer biology, mass spec applications, or cutting-edge cancer research, this...

Join analytical chemist Dr. M. Farooq Wahab from the University of Texas at Arlington as he breaks down the science behind chromatographic peak shapes in this episode of the Concentrating on Chromatography podcast. Perfect for undergraduate chemists and researchers alike, this conversation covers:- What makes an ideal chromatographic peak shape and why it matters - Common reasons your HPLC peaks might show tailing, fronting, or distortion - Practical tips for diagnosing and troubleshooting peak shape problems - How to use visual and digital tools (like Excel) to analyze your chromatogram data beyond basic numbers - The importance of...

Join us for an insightful conversation with Pernilla Sörme, Technical Program Director at My Green Lab, as we explore how one organization is transforming laboratories worldwide into leaders in environmental sustainability. Discover the driving mission behind My Green Lab, the measurable impact on research and industry, and real-world tips for saving energy and reducing plastic waste in scientific labs. Pernilla shares how behavior change, grassroots and leadership buy-in, and a community-driven approach are helping thousands of labs certify their sustainability practices—and why this matters for the future of science, the planet, and organizational success. Whether you’re in ac...

In this episode of the Concentrating on Chromatography podcast, host David Oliva interviews Ainslie Chen about her cutting-edge research on hemoglobin variant testing using liquid chromatography-high resolution mass spectrometry (LC-HR-MS). Ainslie discusses her novel method for separating and identifying hemoglobin subunits with mass shifts less than 1 Da, overcoming critical limitations in conventional clinical assays.Hear firsthand how her team developed an LC-HR-MS workflow using a C4 reversed-phase column, tackled technical challenges (including why C18 columns fell short), and achieved unprecedented sensitivity for variant detection in both adult and neonatal samples. The conversation touches on the potential of mass spectrometry...

Join us for a fascinating conversation with Dr. Subhoja Chakraborty from the University of Central Florida, whose cutting-edge research targets the global challenge of malaria drug resistance. Dr. Chakraborty shares her journey from structure-based drug design during her PhD to her recent collaborative work repurposing human kinase inhibitors as rapid, selective antimalarials against drug-resistant Plasmodium falciparum strains.In this episode, you'll learn:- How structure-guided approaches reveal potential drug targets, including cysteine proteases like falcipain-2, and kinases implicated in malaria's complex life cycle.- The power of repurposing kinase inhibitors originally developed for cancer and other diseases, focusing on...

In this special episode of Concentrating on Chromatography, host David Oliva steps into the guest role to share his journey as featured on the Lab Matters podcast. David discusses his transition from a passion for business and economics to leading Organomation, a renowned manufacturer of laboratory instrumentation and sample preparation tools. Organomation's flagship products—like the N-EVAP, MULTIVAP, and MICROVAP nitrogen evaporators—help scientists accelerate the sample concentration process. These instruments utilize gentle nitrogen gas flow and precise temperature control, enabling labs to prepare sensitive samples with accuracy and efficiency. David explains how these technologies are trusted by academic, gove...

Join us for an exciting deep dive with Isaac Plutzer, a graduate researcher at Washington University in St. Louis, as he explores the mysterious "dark kinome"—the hundreds of under-studied protein kinases with high potential for therapeutic discovery. In this interview, Isaac explains what kinases are and why a substantial fraction remain enigmatic, despite being critical regulators of cellular processes.

Isaac shares how his lab combines innovative proximity labeling and phosphoproteomics techniques with advanced mass spectrometry to systematically map the interactors, substrates, and signaling pathways of these mysterious enzymes. The conversation covers:

What makes a ki...

Join Dr. Guy Caldwell, Distinguished Research Professor at the University of Alabama, for a riveting and urgent conversation that goes beyond the lab bench to the heart of America’s biomedical research crisis. In this episode, Dr. Caldwell—an internationally recognized leader in neurodegenerative disease research—shares the real-world impact of NIH funding freezes, budget cuts, and policy gridlock on labs, promising young scientists, and the future of life-changing discoveries

Hear compelling stories from both the "Worm Shack" lab and classrooms filled with top science students: top-ranked research grants on Alzheimer’s and Parkinson’s now going unfunded...

Join us for a fascinating conversation with Dr. Chad Pozarycki, PhD in analytical chemistry and astrobiology, as we dive into the cutting-edge science behind searching for life beyond Earth. In this episode, we explore groundbreaking research on how biosignature molecules—such as amino acids and ATP—can accumulate and persist in extreme, hypersaline brine environments analogous to those found on Mars, Europa, and other planets.Dr. Pozarycki explains the unique analytical challenges of working with highly salty samples, discusses innovative techniques like capillary electrophoresis and high-resolution mass spectrometry, and offers key advice for scientists developing the next generation of spac...

🧪 Transform Your Understanding of Modern Chromatography & AI!

Join us for an eye-opening conversation with Professor Dwight Stoll from Gustavus Adolphus College as he reveals how his team is revolutionizing liquid chromatography through high-throughput data collection and machine learning. With over 43,000 retention measurements and groundbreaking research spanning three major publications, Prof. Stoll is pioneering the future of analytical chemistry.🔬 What You'll Learn:- How short columns (5mm vs 100mm) dramatically increase measurement throughput while maintaining accuracy- The evolution from HSM1 to HSM3 models and their predictive capabilities - Why selectivity ratios are more stable than absolute retention factors- The "M...

Join David Oliva on the Concentrating on Chromatography podcast as he interviews Edward Faden, Vice President and co-owner of MAC-MOD Analytical, a leading provider of chromatography solutions since 1986. In this insightful discussion, Edward shares the history and expertise of MAC-MOD, based in Chadds Ford, Pennsylvania, and dives deep into the critical challenges of analyzing PFAS (per- and polyfluoroalkyl substances), often called "forever chemicals."Key topics include:- The pervasive nature of PFAS contamination in both environmental and laboratory settings.- Surprising sources of PFAS contamination identified in standard lab setups, from gas line tubing to everyday consumables.- The...

Join us for an in-depth conversation with Dr. Eamonn Clark, postdoctoral researcher at the University of Hawaii,  Mānoa and the Water Resources Research Center, as he shares his journey in water research and laboratory innovation.In this episode:- Dr. Clark introduces his work on water treatment and disinfection byproducts, including research following the Red Hill fuel spill in Oahu.- A behind-the-scenes look at the development of a home-built nitrogen blowdown evaporator—why it was needed, how it was designed, and who can benefit from building their own lab equipment.- Discussion of workflows for PFAS analysis, the i...

Dr. Haleigh Boswell, Research Chemist at Chevron, joins the Concentrating on Chromatography podcast to discuss her fascinating career journey and cutting-edge analytical work in the energy industry. In this in-depth interview, Dr. Boswell shares:🔬 Technical Insights:- How two-dimensional gas chromatography (GCxGC) differs from traditional GC and why it's revolutionary for complex sample analysis- Applications of GCxGC in analyzing hydrocarbon and renewable matrices- Detector selection strategies (FID, SCD, NCD, TOF-MS) for different analytical challenges- Sample preparation considerations for complex energy industry matrices🎯 Industry Applications:- Real-world problem-solving in refinery operations and catalyst development- Non-targeted analysis for trace contaminants in renewa...

In this episode of Concentrating on Chromatography, host David Oliva interviews Dr. Petr Vozka, Assistant Professor at California State University, Los Angeles, about his journey establishing the Complex Chemical Composition Analysis Lab (C³AL).Dr. Vozka shares how he built his research facility during the pandemic, focusing on creating opportunities for first-generation and underrepresented undergraduate students. Learn about the groundbreaking LECO-C³AL partnership that provides students with hands-on experience using advanced comprehensive two-dimensional gas chromatography (GC×GC) instrumentation.The conversation explores:- Building a cutting-edge analytical chemistry lab during challenging circumstances- Creating research opportunities for underrepresented undergraduate students- The inn...

Eugene Oga, a PhD student in Analytical Chemistry at the University of North Dakota, joins David Oliva to discuss his innovative research on silicone sealant degradation using evolved gas analysis and thermal desorption-pyrolysis gas chromatography. With his background in materials chemistry and current focus on analytical techniques, Eugene shares insights into how these advanced methods can characterize material breakdown over time, with significant implications for the construction industry and beyond.Eugene's Background and Research FocusEugene Oga is pursuing his PhD in Analytical Chemistry at the University of North Dakota, following his Bachelor's and Master's degrees from the University of...

In this enlightening episode of Concentrating on Chromatography, host David Oliva engages in a comprehensive discussion with Lisa Jones, Director of Higher Education Relations at I.W. Tremont Co. and active NAOSMM volunteer, alongside Jim Averso, Co-President at I.W. Tremont and producer of the LabExact brand of laboratory consumables.

The conversation explores critical aspects of laboratory supply management, including best practices for procurement in academic settings, inventory challenges, and the advantages of American-made products. Lisa shares valuable insights from her 27 years of experience at the University of Alabama, highlighting how institutional policies affect purchasing decisions and...

In this episode of Concentrating on Chromatography, host David Oliva interviews Jim Averso, Co-President of I.W. Tremont and manufacturer of the LabExact brand, celebrating their 45th year in business. Jim shares expert insights on the critical role of filtration in chromatography sample preparation.Discover:- Why proper filtration is essential for protecting your expensive HPLC columns- How to select the right membrane type (Nylon, PTFE, PVDF, or PES) based on your sample- Common mistakes researchers make with syringe filters and how to avoid them- The innovative TruPor membrane technology that increases loading capacity while improving flow rates- Tips...

Join us on the Concentrating on Chromatography podcast as David Oliva interviews Katelynn Perrault Uptmor, PhD about her recent article, "Detangling the Complex Web of GC x GC Method Development to Support New Users." In this insightful conversation, Katelynn explains the basics of comprehensive two-dimensional gas chromatography (GC x GC), its advantages over traditional one-dimensional GC, and shares practical tips for overcoming common challenges faced by new users.Discover how GC x GC vastly increases separation capacity by combining two columns with different stationary phases, allowing for more efficient analysis of complex samples. Katelynn discusses the key steps in...

Dive into the world of cutting-edge catalysis research with Dr. Roberto Silva Villatoro in this captivating episode of Concentrating on Chromatography. Hosted by David Oliva, General Manager of Organomation, this interview explores how high-resolution mass spectrometry (HRMS) is revolutionizing our understanding of palladium-catalyzed reactions. Key highlights: - Discover how HRMS detects elusive organometallic intermediates - Learn about overcoming air-sensitivity challenges in sample preparation - Explore the implications of off-cycle species in catalytic processes - Gain insights into the future of C-X and C-C bond-forming reactions Whether you're a chemistry enthusiast, a seasoned researcher, or an industry professional, this interview...

Join us for an insightful interview with Dr. Isaiah Speight, Assistant Professor at William and Mary, as he discusses his groundbreaking work in sustainable chemistry and innovative chromatography techniques. In this engaging conversation, Prof. Speight shares: - His research focus on sustainable chemistry from synthesis, financial, and educational perspectives - The importance of NOBCChE (National Organization for the Professional Advancement of Black Chemists and Chemical Engineers) - How HPLC and flash chromatography enable synthetic chemistry - The advantages of automated flash chromatography systems - Method development processes for new compounds and reactions - Exciting ongoing projects, including 3D-printed mechanochemical...

Dive into the world of environmental toxicology with Andrea Andress Huacachino, a PhD student at the University of Pennsylvania. In this fascinating interview, Andrea discusses her groundbreaking research on PFAS (per- and polyfluoroalkyl substances) and their impact on androgen regulation. Learn about: - The novel finding of PFOA's effect on pre-receptor androgen regulation - Innovative use of radio chromatography in hormone analysis - Challenges in developing HPLC methods for androgen separation - The broader implications of androgen dysregulation on human health Discover how environmental pollutants affect hormones in all humans and why this research matters for everyone. Perfect for...

Dr. Caitlin Cain discusses her groundbreaking research on potato taste defect (PTD) in coffee using comprehensive two-dimensional gas chromatography (GC×GC). Learn how this advanced technique revealed 359 class-distinguishing analytes related to PTD, providing unprecedented insights into this complex flavor issue. Key points covered: - Advantages of GC×GC over traditional one-dimensional GC for complex samples - Optimization of headspace solid-phase microextraction for coffee volatile analysis - Surprising findings from the comprehensive volatile profile of PTD-affected coffee - Implications for coffee farmers and the industry in combating PTD Discover how this research is advancing our understanding of coffee chemistry and po...

Dive into the cutting-edge world of natural product discovery with Jess Deng, a fourth-year PhD candidate at Queen's University. In this engaging interview, Jess discusses her groundbreaking research using ambient ionization mass spectrometry to streamline the discovery of novel fungal metabolites. Key highlights: - Learn about the liquid microjunction surface sampling probe (LMJ SSP) and its advantages - Discover how Jess is tackling the time and resource challenges in natural product research - Explore the effects of light conditions and environmental factors on fungal metabolism - Hear about Jess's exciting work at the Smithsonian Museum of Conservation From rapid...

In this insightful interview, Medicinal Chemist Schyler Odum discusses his work in antibiotic drug discovery and the critical role of Ultra-High Performance Liquid Chromatography (UPLC) in his research. Schyler, a fifth-year graduate student at UTHSC, shares his expertise on: The importance of antibiotic drug discovery in academic institutions How UPLC differs from HPLC and its benefits in chemical analysis Challenges in sample preparation and potential improvements The complexities of synthesizing large molecular weight antibiotics The need for increased support and funding in antibiotic research Schyler also highlights the Gordon Research Conferences as a platform for presenting antibiotic drug discovery...

Dive into the world of sample preparation with industry expert Wade Hasenour as he shares invaluable insights on rotary evaporation, centrifugal evaporation, and freeze drying. Learn about the latest advancements, challenges, and future trends in sample prep techniques for chromatography. Discover how to choose the right method for your needs, optimize your workflow, and leverage automation for better results. Whether you're a seasoned chromatographer or just starting out, this video offers essential knowledge to enhance your sample preparation skills and achieve more reliable outcomes in your research. #Chromatography #SamplePreparation #LabTechniques #ScientificResearch #massspectrometry

Dive into the cutting-edge world of pharmaceutical analysis with Mark Dennis Retrato, a PhD candidate at Uppsala University. In this exclusive interview, Mark shares his groundbreaking research on developing a novel UPLC-MS method for analyzing phospholipids in total parenteral nutrition (TPN) products. Key highlights: • Innovative UPLC-MS method for phospholipid quantification • Importance of nitrogen evaporation in sample preparation • Unexpected findings in phosphatidylcholine species • Implications for quality control in pharmaceutical manufacturing • Potential impact on patient care and TPN product stability Learn how this research is pushing the boundaries of analytical chemistry and contributing to improved healthcare outcomes. Whether you're a scientist, healthcare...

Join Organomation's General Manager David Oliva as he interviews Bianca Costa, a PhD researcher from the University of Southern California, about her groundbreaking work on PFAS (per- and polyfluoroalkyl substances). In this in-depth discussion, Bianca shares her experiences and insights on: • The complexities of PFAS sampling and analysis in various environmental matrices • Challenges in method development for PFAS extraction and detection • The use of advanced analytical techniques like LC-MS for PFAS research • Emerging biotechnologies for PFAS remediation and the potential for microbial transformation • Community engagement efforts to assess PFAS exposure in Southern California Discover the latest advancements in PFAS research a...

Join us for an interesting interview with Taylor Bias, a leading researcher in the field of mitochondrial proteins, as she discusses her groundbreaking work on mitoNEET and its implications for diseases like Type 2 Diabetes, Parkinson's Disease, and Cancer. In this engaging conversation with Organomation General Manager David Oliva, Taylor shares the challenges she faced in developing analytical methods to study mitoNEET's enzymatic activities and the pivotal role of chromatography techniques like GC-MS and HPLC in her research. Discover how chromatography is evolving in the study of complex proteins and hear about some unexpected findings that have emerged from Taylor's...

In this episode of "Concentrating on Chromatography," host David Oliva explores diverse applications of chromatography and mass spectrometry with researchers from the University of Nevada, Reno. The discussion covers a range of topics, including:

Listeners will gain insights into cutting-edge research methodologies, sample preparation techniques, and the critical role of chromatography in environmental science, microbiology, animal welfare, and beyond. This episode...

In this insightful interview, David Oliva, General Manager of Organomation, sits down with Jonathan Navarro Ramos, a PhD student at the University at Buffalo, to discuss his cutting-edge research on environmental pollutants. Jonathan shares his journey in developing methods to detect tire-derived chemicals like 6PPD and 6PPD-quinone in stormwater runoff and highlights the importance of non-target analysis using high-resolution mass spectrometry (HRMS). Key Topics Covered: Research on 6PPD and its transformation into 6PPD-quinone Environmental and public health concerns surrounding tire-derived chemicals The use of LC-MS/MS in detecting pollutants in challenging matrices Sample preparation techniques, including solid-phase extraction and...